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Single-cell Immunogenomics Data Analysis Tutorial

Author: Xin Liu
Time: 9 min
Words: 1.8k words
Updated: 2025-11-07
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SeekSoulOnline User Guide scRNA+VDJ Single-cell Immunogenomics

Getting Started

Start your analysis after logging in to SeekSoulOnline.

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Publication

Databases

Centrally store and manage user sample data and functional gene sets so they can be referenced directly in analysis workflows.

My Data

Manage your sample data with support for purchasing and uploading.

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My Database

Collect curated functional gene sets for differential enrichment analyses.

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My Gene Sets

Collect curated gene sets for annotation and scoring analyses.

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Settings

SeekSoulOnline supports configuring parameter templates and updating personal information.

Parameter Templates

  • Customize analysis parameters
  • Customize color templates
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Profile

  • Account information
  • Change password
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Standard Analysis

SeekSoulOnline provides single-cell experiment quality control and standardization knowledge base and tools to help researchers achieve precise control and standardization in sample preparation and quality evaluation stages.

Basic Analysis

  • Data preprocessing and quality control
  • Dimensionality reduction and clustering analysis
  • Basic visualization methods
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Cell Annotation

  • Cell type identification methods
  • Marker gene analysis
  • Annotation strategies and techniques
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Plotting Tools

  • Introduction to various plot types
  • Parameter settings and optimization
  • Plot export and sharing
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Feature Analysis

  • Gene set activity analysis
  • Cell function scoring analysis
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Differential Enrichment

  • Differential gene identification
  • Enrichment analysis
  • GSEA analysis
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Subgroup Selection

  • Filtering subgroup data
  • Filtering cells
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Advanced Analysis

  • Cell communication analysis
  • Pseudo-time series analysis
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Interactive Analysis

  • Dimensionality reduction visualization
  • Modifying labels
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Auxiliary Information

  • Data download (permission)
  • Metadata management
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Result Overview

  • Result summary display
  • Result export and sharing
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Smart Support

SeekSoulOnline's single-cell experiment experience, cell annotation, and common problem AI intelligent expert.

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寻小因

Document Center

This center collects standardized documents from experiment preparation, sequencing to data analysis, aiming to provide clear and accurate operation guides and theoretical support for your research projects.

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Datasets
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