Outputs
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ARC Multi-omics Analysis Outputs
The main output directory structure for ARC multi-omics analysis is shown below. Each line represents a file or directory, and the numbers indicate important output files. demo is the example sample name.
shell
./
├── outs
│ ├── filtered_feature_bc_matrix 1
│ │ ├── barcodes.tsv.gz
│ │ ├── features.tsv.gz
│ │ └── matrix.mtx.gz
│ ├── filtered_peaks_bc_matrix 2
│ │ ├── barcodes.tsv.gz
│ │ ├── features.tsv.gz
│ │ └── matrix.mtx.gz
│ ├── raw_feature_bc_matrix 3
│ │ ├── barcodes.tsv.gz
│ │ ├── features.tsv.gz
│ │ └── matrix.mtx.gz
│ ├── raw_peaks_bc_matrix 4
│ │ ├── barcodes.tsv.gz
│ │ ├── features.tsv.gz
│ │ └── matrix.mtx.gz
│ ├── demo_A_fragments.tsv.gz 5
│ ├── demo_A_fragments.tsv.gz.tbi 6
│ ├── demo_A_mem_pe_Sort.bam 7
│ ├── demo_A_peaks.bed 8
│ ├── demo_E_SortedByName.bam 9
│ ├── demo.rds 10
│ ├── demo_report.html 11
│ └── demo_summary.csv 12
└── analysis- Cell-filtered sparse gene expression matrix.
- Cell-filtered sparse ATAC Peak matrix.
- Raw sparse gene expression matrix.
- Raw sparse ATAC Peak matrix.
- ATAC fragment information file.
- Fragment file index.
- ATAC library alignment BAM file.
- ATAC Peak file.
- RNA library alignment BAM file.
- Seurat object and downstream analysis results in RDS format.
- Analysis report in HTML format.
- Quality-control statistics in CSV format.
RNA-only Library Analysis Outputs
The main output directory structure for RNA-only library analysis is shown below. demo is the example sample name.
shell
./
├── analysis
│ └── demo_E
│ ├── step1
│ │ ├── demo_E_1.fq.gz
│ │ └── demo_E_2.fq.gz
│ ├── step2
│ │ └── featureCounts
│ │ └── demo_E_SortedByName.bam 1
│ ├── step3
│ │ ├── raw_feature_bc_matrix 2
│ │ │ ├── barcodes.tsv.gz
│ │ │ ├── features.tsv.gz
│ │ │ └── matrix.mtx.gz
│ │ ├── filtered_feature_bc_matrix 3
│ │ │ ├── barcodes.tsv.gz
│ │ │ ├── features.tsv.gz
│ │ │ └── matrix.mtx.gz
│ │ ├── counts.xls
│ │ ├── detail.xls
│ │ └── umi.xls
│ └── step4
│ ├── demo.rds 4
│ ├── gex_tsne_umi.xls 5
│ └── gex_FindAllMarkers.xls 6
└── outs
└── demo_summary.csv 7- RNA library alignment BAM file.
- Raw RNA expression matrix.
- Cell-filtered RNA expression matrix.
- Seurat object and downstream analysis results in RDS format.
- Cell embedding coordinates, UMI counts, mitochondrial proportion, and related information.
- Marker gene table generated from Seurat clustering results.
- RNA/GEX quality-control statistics.
