Dataset 4（SeekOne DD scFAST-seq platform）
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Words: 222 words
Updated: 2025-07-14
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Sample Source：
Three cell lines mixed: A549：KRAS G12S；HCC827 EGFR 19del, K562: BCR-ABL1 fusion (1:1:1)
Experimental Method：
SeekOne™ DD Single-Cell Full-length RNA Sequence Transcriptome-seq Kit was used to perform single-cell separation, nucleic acid labeling, and capture on the SeekOne™ Digital Droplet System. The high-throughput single-cell transcriptome libraries were sequenced using the Illumina NovaSeq 6000 platform.
Data Analysis：
Raw sequencing data was converted to fastq format with Bcl2fastq, then low-quality data was filtered out using the fastp quality filtering software. The filtered sequencing results were analyzed with the SeekSoul Tools, generating intermediate files like single-cell library quality control results, expression matrices, and alignment results.
Data Download：
Analysis Report: Download
Expression Matrix: matrix: Download
Expression Matrix: RDS: Download
Raw Data: Download
- md5: 878de2833feea2deb7d79224409d0d09 (Please be patient, raw data is large and may take some time to download)
Panel Raw Data: Download
- md5: a99ba979b0c6ae6465539f2ce57ad5c2 (Please be patient, raw data is large and may take some time to download)